We surveyed 2311 conservation professionals working across 122 countries, asking about experiences of psychological distress, working conditions, and personal characteristics. Over half were from and worked in Europe and North America, and most had university-level education, were in desk-based academic and practitioner roles, and responded in English. Heavy workload, job demands, and organizational instability were linked to higher distress, but job stability and satisfaction with one's contributions to conservation were associated with lower distress. Our results flag important risk factors that employers could consider, though further research is needed among groups under-represented in our sample. We suggest ways employers and others might promote the positives and manage the risks of working in the sector, potentially supporting conservationists’ mental health and abilities to protect nature. https://doi.org/10.1111/cobi.14097